Iron overload in β- Thalassemia major induced mostly by regular blood transfusions.The in vitro study was designated for Iron chelation and antioxidant activity of Zataria multiflora and Matricaria chamomilla plant extracts in thalassemic and healthy serums. Materials and Methods: Iron chelation, antioxidant activity, total phenol and flavonoids contents of Zataria multiflora and Matricaria chamomilla plant extracts were determined. For in vitro study of iron chelating, plant, extracts were exposed with serum thalassemic for 2h. Malondialdehyde (MDA) in blood serum and Superoxide Dismutase(SOD) in red blood cells were determined. Serum iron before and post expose with plant extracts was determined. Results: There was significant increase in iron concentration in β - thalassaemia major compare to control serums. Iron level in thalassemic and control serums were reported 178 ±8.2 and 89 ± 12 respectively. Iron concentration in thalassemic serums after exposed with Z.multiflora and M. chamomilla extracts in 2 hour incubation at in vitro state were decreased 27 and 33 % respectively. However, iron content in serum controls after expose with Z. multiflora and M. chamomilla extracts were decreased 16.8% and 23.6 % respectively. Conclusion: MDA and iron in blood serum and SOD in erythrocyte of thalassemic samples was significantly higher than healthy persons. However serum vitamin E was lower than healthy person.
کلید واژگان :β- Thalassemia major,iron chelating, Zataria multiflora, Matricaria chamomilla
ارزش ریالی : 600000 ریال
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