بررسی نقش محافظتی سیلدنافیل در برابر تغییرات ناشی از ترومای غیرنافذ تجربی یک طرفه بیضه در بافت، خصوصیات اسپرم و توان باروری آزمایشگاهی (IVF) بیضه طرف مقابل موش سفید کوچک آزمایشگاهی

Unilateral blunt testicular trauma (UBTT) occurs most commonly as a result of sport events, and can range from contusion to complete rupture testicle. It has been reported that high incidence of remote testicular trauma in infertile men. The aim of this study was to investigate the protective effects of sildenafil (SF) as an antioxidant and anti-inflammatory agent against unilateral blunt testicular trauma in mice. Thirty-six immature male mice were randomized in six equal groups. Group1: control, group 2: UBTT, group 3: UBTT+ SF, group 4: Vit.E +UBTT, group 5: SF and group 6: Vit.E. To induce trauma, the abdomen was opened and the left testis was placed on a sterile firm surface and 5 g sterile weight was dropped on to the testis from a height of 10 cm. At the 70 days of age, right testis and sperms from cuda epididymis were harvested to evaluate in vitro fertilizing capacity and sperm and histological characteristics. UBTT led to significant decreases in the weight of contralateral testis and spermatogenic indices along with severe histomorphologic alterations in testicular tissue. In addition, UBTT resulted in significant decrease in sperm motility and fertilization rate with an increase in sperm mortality and abnormality. Administration of SF following the trauma, improved the above-mentioned parameters. This study suggests that UBTT can adversely damage the contralateral testis through disturbing blood-barrier-testis, provoking immunological responses and reactive oxygen species overproduction, while sildenafil can improve these adverse effects by effective inhibiting of oxidative and inflammatory processes.