Despite a large number of investigations on the molecular genetics and population structure of the whiteXy Bemisia tabaci (Gennadius) complex, no such study had been conducted in Iran. The genetic variation of B. tabaci was examined using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) for 18 Weld collections from cucumber, eggplant, and tomato in four provinces of Iran. PCR ampliWcation and restriction digestion with two enzymes detected 388 RFLP fragments, of which 16 fragments showed polymorphisms. Cluster analysis of these data placed all B. tabaci individuals within a single group, and there was no evidence for between- or within-population genetic variation. Phylogenetic (Clustal W) analysis of 42 B. tabaci mtCOI sequences (n = 21 Weld collections) from Iran, and a comparison with well-studied haplotype or biotype reference sequences available in public sequence databases, revealed that the Iranian B. tabaci populations were most closely related to the B biotype at 0–1.2% nucleotide identity. The B biotype is a well-known member of a sister clade from the Middle East–North African region of the world, owing to its nearly worldwide distribution and invasive characteristics. This report indicates that a single major haplotype of B biotype is prevalent in Iran and that its closest relative is the B biotype. Also, given the extent of known variation in the Middle East and African continent, data indicate somewhat surprisingly that the B. tabaci collections sampled in Iran had limited genetic variation and population substructure. Knowledge that the B biotype of B. tabaci predominates in Iran is important for designing eVective pest management strategies given that biotypes of B. tabaci are known to diVer greatly with respect to insecticide resistance, host range, virus–vector interactions, and other key biological characteristics.
کلید واژگان :Bemisia tabaci · Genetic variation · Iran · mtCOI · PCR-RFLP · Phylogeny
ارزش ریالی : 600000 ریال
با پرداخت الکترونیک