Brucellosis is a zoonotic disease which is characterized by reduced fertility and abortion in several species of animals and human. Camel brucellosis is caused by Brucella abortus and Brucella melitensis. To overcome the limitations posed by other techniques such as culture and serology, a sensitive technique (PCR) was employed in this study for detection of camel brucellosis. One hundred twenty three camels were used to study brucellosis infection by using Polymerase Chain Reaction (PCR) method. Findings from this study indicated that, 11.38% (n=123) of blood samples were positive for Brucella spp. and 13.01% (n=123) of the lymph node samples were positive for Brucella spp. by PCR method. In this study, 5 out of 123 (4.065%) and 3 out of 123 (2.439%) camel blood samples were positive for B. abortus and B. melitensis, respectively using PCR. Also, 4 out of 123 (3.252%) and 2 out of 123 (1.626%) camel lymph node samples were positive for B. abortus and B. melitensis, respectively using PCR. Young camels were the most common infected age group, while adult camels were the less common infected age group. Also, higher prevalence of brucellosis was observed in female camels. These results have indicated that PCR is a sensitive technique which could be used as a confirmatory test for the detection of brucellosis in live camels by the lowest risk of infection to laboratory personnel. Also, suggested that control and eradication programs for Brucella spp. infection seems to be necessary in camels. Our findings support the power of PCR test for Brucella spp. detection in blood and lymph node samples and could be easily used for routine diagnosis.
کلید واژگان :Camel, Brucellosis, zoonosis, blood, lymph nodes, Polymerase Chain Reaction (PCR)
ارزش ریالی : 600000 ریال
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