Leptospira is an emerging global public health problem, which involves both animals and human. The aim of this study was to appraise the presence of Leptospira DNA in blood samples from cattle and sheep via Polymerase Chain Reaction (PCR) method in Chaharmahal Va Bakhtiari and Isfahan provinces of Iran. Of 195 samples (92 blood sample of cattle and 103 blood sample of sheep) were collected randomly from clinical healthy animals. DNA was extracted from the blood samples and stored at −20 °C before examination. PCR reaction was performed for detection of Leptospira DNA using specific primers for 16s rRNA gene and PCR products were visualized in a 1% agarose gel electrophoresis. The results showed that 18.63% of blood samples were positive. Leptospiral DNA was found in 20 of 92 (21.73%) and 16 of 103 (15.53%) of cattle and sheep blood samples, respectively. Was found 9 (9.78%) out of the 92 cattle sampled and 5 (4.85%) out of the 103 sheep sampled were positive to Leptospira spp. serovar Hardjo. This indicated that these cows and sheep's are reservoirs and dangerous for human health. PCR method is sensitive and specific for diagnosis of Leptospira in suspected cases.
کلید واژگان :blood, cattle, Leptospirosis, PCR, prevalence, sheep
ارزش ریالی : 600000 ریال
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