Objective: To investigate virulence associated characteristics in uropathogenic Escherichia coli (E. coli) isolates derived from urine specimens, and to investigate the distribution of the pathogenicity islands virulence markers (PAIs) among the isolates. Methods: In this study, 63 E. coli strains isolated from patients with symptoms of urinary tract infections were collected and all of them were confirmed by biochemical tests and molecular techniques. PCR was conducted by specific pair primers in order to determine the prevalence of pathogenicity islands markers. Results: Among the 63 examined isolates, PAI IV536 was reported in 53 strains (84.12%) showing the highest prevalence. The distribution of PAI IICFT073, PAI III536, PAI I536, PAI II536, PAI J196, PAI IIJ196, PAI ICFT073 were 41.26%, 4.76%, 34.92%, 20.63%, 1.57%, 31.74% and 72.01%, respectively. Conclusions: Pathogenicity island markers cause horizontal transfer of genes. Many virulence genes are present in the pathogenicity islands regions in such a way that by deletion and/or mutation of these genes, the bacteria would lose its pathogenicity. Knowledge of the molecular details of uropathogenic E. coli is useful to develop successful strategies for the treatment of urinary tract infection and complications associated with urinary tract infections in human.
کلید واژگان :Escherichia coli Urinary tract infection Pathogenicity island markers PCR
ارزش ریالی : 600000 ریال
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