During spring and summer of 2011, a survey was undertaken on some palm groves in the Kerman province (south-eastern Iran) to determine the fungal pathogens associated with date palm (Phoenix dactylifera L.) decline diseases. Samples were taken from date palm trees showing yellowing, wilting and dieback symptoms. Isolations were made from symptomatic tissues on malt extract agar (MEA) supplemented with 100 mg/l streptomycin sulphate (MEAS). Two species of Phaeoacremonium, Phaeoacremonium aleophilum and Pm. parasiticum, and two species of Botryosphaeriaceae, Botryosphaeria dothidea andDiplodia mutila, were isolated from affected trees and identified on the basis of morphological, cultural and molecular characteristics. Pathogenicity tests were performed on date palm (4-year-old potted plants) under greenhouse conditions. Based on the pathogenicity tests, Pm. aleophilum was the most virulent and caused the longest lesions. This is the first report of Pm. aleophilum and B. dothidea and their pathogenicity on date palm tree.
کلید واژگان :internal transcribed spacers, pathogenicity test, Vitis vinifera, b-tubulin
ارزش ریالی : 600000 ریال
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