Objective: In order to gain a better understanding of ewes pregnancy physiology and fetal metabolism, the present study was carried out to determine the values of amniotic fluid (AF) progesterone and biochemical constituents and its associated with placenta traits in Iranian crossbred ewes (Arkhar-Merino×Ghezel). Methods: The sixty ewes (2-5 years old, weighing 40-50 kg) were treated with CIDR induced 30 mg progesterone for 14 days and were injected 400 IU PMSG at the time of CIDR withdrawal. After the detection of estrus, ewes were hand-mated. After expelling of each fetus accompanied by fetal membranes, 10 ml AF was taken from fetal sac for spectrophotometer methods (glucose, urea, creatinine, total protein, cholesterol, triglycerides, calcium and phosphorus) and radioimmunoassay (progesterone) analysis. Results: Results indicated that highly positive correlations between placental efficiency and cotyledon density existed (r= 0.764, p<0.01), and negative correlation between placenta weight (PW) with the total volume amniotic fluid (TVAF) (r=−0.872, p<0.01). A positive relationship was calculated between TVAF with total protein and creatinine (r=0.418, p<0.01 and r=0.639, p<0.05, respectively). However, the correlation between the glucose and PW, cotyledon length and calcium were significantly positive (r = 0.704 and r = 0. 712; p<0.01, respectively). The findings of this study demonstrated that there were no relationships between progesterone concentrations and placental traits (p>0.01), except for PW (r=0.665). Conclusions: In conclusion, the significant correlation between the AF biochemical and progesterone with placental traits for the above-mentioned metabolites suggests that metabolic changes in AF levels will be reflected the condition in the AF.
کلید واژگان :Biochemical, Crossbred ewes, Amniotic Fluid, Placenta Traits, Progesterone
ارزش ریالی : 600000 ریال
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