Abstract. – BACKGROUND: Rotavirus infection is the most common cause of gastroenteritis among children worldwide. In this study, we were evaluated the occurrence of rotavirus infection and genotypes causing gastroenteritis among children under 5 years inYasuj, Iran. MATERIALS AND METHODS: This cross sectional- descriptive study was done on 184 fecal samples collected from children aged < 5 years old with acute gastroenteritis. All the stool specimens were tested for group A rotavirus with enzyme immunoassays (EIA). Rotavirus-positive specimens were genotyped by the Nested reverse transcription polymerase chain reaction (RT-PCR) with using different type of specific primers. RESULTS: The prevalence of rotavirus was 28.26% and predominant genotypes were G1 (1.92%), G2 (7.69%), G4 (1.92%), G8 (46.16%), N.T.A (40.39%), mixed infections (1.92%). Predominance of rotavirus G8 genotype in the current survey was reported for the first time in Iran. The highest prevalence of rotavirus infection was observed in autumn (48.08%), and the lowest in spring (5.77%). CONCLUSIONS: The result of study showed that prevalence of rotavirus genotypes in this region of the country is different from that of other regions. The continuous stability of rotavirus in Iran, regarding to the regional differentiates and prevalence of circulating strains, can be useful indication in order to supply and effective vaccine against the rotavirus infections.
کلید واژگان :Rotavirus, Gastroenteritis, Children, Genotype, Nested RT-PCR.
ارزش ریالی : 600000 ریال
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