This study investigated the use of ultrasoundassisted extraction (USAE) to improve the extraction efficiency of the classical solvent extraction techniques such as maceration and Soxhlet extraction to extract three coumarin compounds (osthol, isoimperatorin and oxypeucedanin) from dried roots of Prangos ferulacea (L.) Lindl. Separation was accomplished on a reverse-phase C18 column with the mobile phase of methanol–water (70:30) and detection of the analytes was performed with UV variable detector at a wavelength of 322 nm. Under the optimum conditions, the extracted amounts of osthol, isoimperatorin and oxypeucedanin were 157.66, 172.54 and 79.27 mg g−1 dry herb, respectively. The results also indicated that compared with Soxhlet at 8 h and maceration extraction method at 3 days, ultrasound-assisted extraction increased the extraction yield of the total coumarin compounds about 17 and 31 %, respectively, at only 30 min. Therefore, USAE was preferred as the most efficient method which consumed less amount of a nontoxic solvent at a short time in comparison with conventional methods for extraction of coumarin compounds.
کلید واژگان :Ultrasound-assisted extraction · Prangos ferulacea · Coumarin compounds · Osthol, isoimperatorin · Oxypeucedanin
ارزش ریالی : 600000 ریال
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