Background: Latex agglutination test for Kala-azar (KAtex) is an easy, inexpensive, and field-applicable antigen de-tection test. However, the main drawback of this method is the boiling step applied to remove false positivity of the test. This study was conducted to survey false positivity results of latex agglutination test for KAtex without boiling process in urine of some autoimmune patients. Methods: Ninety-two urine samples from autoimmune patients including systemic lupus erythematosus (SLE), rheu-matoid arthritis (RA), scleroderma, autoimmune vasculitis, vitiligo, pemphigus and Wagner cases and 20 urine samples from healthy individuals were collected from Kerman Province in Southeastern Iran in 2010-2011. All urine samples were checked by KAtex after boiling for 5 min false positivity rate of the test was surveyed in different healthy and patients groups while boiling process was removed. Rheumatoid factor (RF) then was checked in sera of all cases to evaluate the relationship between RF and KAtex false positivity. Results: All samples represented negative results with KAtex when boiling was performed (100% specificity). Then, 20% positivity was evident in healthy cases. False-positive reactivity was more prominent observed in patient groups than healthy individuals, except in vitiligo. However, a significant difference was only observed in RA group (P<0.05). RF was related to KAtex false positivity. Conclusion: RA was described as the autoimmune disease in which KAtex false positivity was higher than normal population. RF or its metabolic products may have role in false positivity of KAtex but this finding needs to be con-firmed by more reliable and improved experiments. Overall, immune system products should be considered in at-tempts for modification of KAtex for boiling process removal.
کلید واژگان :False positivity, KAtex, Rheumatoid factor, Autoimmune patients
ارزش ریالی : 600000 ریال
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