Escherichia coli (E. coli ) is one of the most common hospital infections in which the emergence of antibiotic resistance and the prevalence of Extended Spectrum Beta-Lactamase (ESBL) are considered major problems in the control of hospital infections. The aim of this study was to investigate the frequency of blaCTX-M, blaTEM, and blaSHV beta-lactamase genes in E. coli isolated from different clinical samples by the combined disk and multiplex PCR methods. E. coli isolates from different clinical samples were collected over six months and identified with the help of standard microbiological and biochemical tests. The antibiotic resistance of E. coli isolates was determined by the disk diffusion method. The production of beta-lactamases was evaluated by the combined disk method, and the presence of blaCTX-M, blaTEM, and blaSHV genes was assessed by the multiplex PCR method. From the total of 200 examined samples, 114 E. coli isolates (57%) were identified with the highest resistance to ceftazidime, amikacin, cefotaxime, and tetracycline (100%) and the lowest resistance to imipenem (3.5%) and ampicillin (14%). 58 isolates (50.88%) were ESBL positive by phenotypic test, of which 20 isolates (34.48%) contained the blaTEM gene and 15 isolates (25.86%) contained the blaCTX-M gene. No positive blaSHV gene was observed in the samples. The presence of beta-lactamase genes examined in this study along with the occurrence of antibiotic resistance indicates the spread of bacterial resistance in the study area and shows the importance of rational antibiotic treatment.
کلید واژگان :blaCTX-M, blaTEM, blaSHV, Escherichia coli, Beta-Lactamase
ارزش ریالی : 600000 ریال
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