Background: Various prokaryotic and eukaryotic expression systems have
been developed for the production of recombinant proteins. In the present
study, we used a new protein expression system based on the Iranian Lizard
Leishmania, a trypanosomatid protozoan as a host, for the expression of LPG3
gene from Leishmania infantum (L.infantum).
Methods: The LPG3 gene was cloned in the expression cassette for integration
into the small subunit of the ribosomal RNA locus of Lizard Leishmania genome
by electroporation. Expression of the recombinant LPG3 protein was
confirmed by western blotting and immunofluorescence staining.
Results: Western blotting confirmed the expression and production of rLPG3
protein. Immunofluoresence analysis also revealed the staining throughout the
cytoplasm of transfected parasites, indicating that the protein has been expressed.
Conclusion: These results demonstrate that Leishmania cells can be suggested
an expression system for the production of recombinant LPG3 (rLPG3) to further
research in vaccine designing against leishmaniasis.
