In this work, the effects of two micro nutrients (Cu2+, Mn2+) on plant growth and accumulation of essential oils in shoot cultures of Lemon balm (Melissa officinalis L.) were investigated. Of the two micro nutrients, copper was more effective in stimulating the accumulation of Caryophyllene β, Citronellal, Geranial, Geraniol, Geranyl Acetate, Linalool and Neral. At 150 ppm, micro nutrients enhanced the production of citronellal, Chavicol. Although combination of Cu2+and Mn2+ at 300 ppm in some of essential oils like neral, e-caryophyllene, caryophyllene oxide and 14-hydroxy-Z-caryophyllene were more produced than 150 ppm combinations but most of essential oils significantly increased in 150 ppm concentration of micronutrients. Trans-piperitone epoxide was upper extracted in 300 ppm concentration of Mn2+ but in many combination this essential oil was no extracted in little concentration. Geranial, Geraniol, (E-) Caryophyllene, Caryophyllene oxide and Neral were the main components in all treatments.
کلید واژگان :Medicinal plant, Melissa officinalis L., Micronutrients, Phytochemical
ارزش ریالی : 600000 ریال
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