Antibiotic Resistance Pattern and Prevalence of blaOXA-51, blaNDM, blaVIM, blaPER, blaVEB, blaCTX, tetA and tetB Genes in Acinetobacter baumannii Isolated from Clinical Specimens of Hospitals in Tabriz city, Iran

Background: Nowadays, the resistance of pathogenic bacteria to antibiotics has become a global problem. Acinetobacter baumannii is an important opportunistic nosocomial pathogen. Acinetobacter baumannii plays a significant role in antibiotic resistance. Objectives: The purpose of this study was to investigate the prevalence of the blaOXA-51, blaNDM, blaVIM, blaPER, blaVEB, blaCTX-M, tetA and tetB genes and antibiotic resistance pattern of A. baumannii isolated from hospitals in Tabriz city, Iran. Methods: This study was descriptive cross-sectional research, performed on 129 isolates of Acinetobacter from different clinical specimens. The Isolates were identified using standard laboratory methods and culture in selective mediums. The antibiotic resistance pattern of isolates was also determined by the Kirby-Bauer disk diffusion susceptibility test. Phenotypic and genotypic detection of blaOXA-51, blaNDM, blaVIM, blaPER, blaVEB, blaCTX-M, tetA and tetB genes in the isolates was carried out by a combined disk test (CDT) and polymerase chain reaction (PCR), respectively. Results: The highest resistance of isolates was determined to cefotaxime (100%) and ceftazidime (100%). The results of CDT showed that 14 (12.96%) isolates could produce extended-spectrum Beta-lactamases (ESBLs). However, the PCR results blaOXA-51, blaNDM, blaVIM, blaPER, blaVEB, blaCTX-M, tetA and tetB genes showed that these genes were in 100%, 18.51%, 16.66%, 32.40%, 16.66%, 31.48%, 32.40% and 21.29% of isolates, respectively. Conclusions: Due to the high prevalence of antimicrobial resistance in strains, rapid and timely detection of antibiotic-resistant A. baumannii strains is necessary for the selection of an appropriate therapeutic approach and prevention of their prevalence.

Acinetobacter baumannii, Beta-lactamase Gene, Antibiotic Resistance, PCR