Effect of Silver Nanoparticles and Hydro Alcoholic Extract of Cucurbita Pepo on Histopathological Changes from Skin Burns in Mice

Introduction: The antimicrobial and anti-inflammatory nature of silver nanoparticles is the most exploited nature of silver nanoparticles in the medical field. But there is concern that excessive use of these materials potentially hazardous to ecosystems, human life and other living organisms should be followed. Materials and methods: In 72 mice at first the burn area 1/5 ×1/5 was created and then divided into nine groups (8 males /group) of control, positive control, 3 groups received 125,250and 500PPm dose of nano silver, 3 groups received different concentration of hydro alcoholic extract of Cucurbita Pepo 25%, 50% , 70% and at the latest a group received pumpkin 25% with 125PPm nano silver. After the end of treatment samples with hematoxylin - eosin were stained. Resules: The highest rate of wound healing in the group receiving the extract is Cucurbita Pepo 50% so that in the end only in this group have maintained normal structure of epidermis and dermis. In the group receiving the nanoparticle dose PPm125 the best results but other groups besides being improved epidermoid cysts also have been created. Conclusion: Heat shock caused by burns can lead to increased free radicals. On the other hand, silver nanoparticles can also cause the production of free radicals So Cucurbita Pepo extract can be effective in reducing these complications.

Burn injury, Skin, Nanosilver, Cucurbita pepo.