Compensatory Role of vitamin E on Testis Tissue and Sperm parameters in Mice Treated with Phenylhydrazine

Background: Hemolytic anemia and consequently the reduction of oxygen can because the deleterious effects on testis function and Spermatogenesis. Accordingly, the iron released form RBC and following that increasing the iron in tissue may exerts Stress Oxidative. Therefore, this survey was done with the aim of reducing harmful effects of hypoxia on reproductive organs. Present study was carried out to evaluate the protective role of vitamin E on testis and sperm parameters under Oxidative Stress conditions due to hemolytic Anemia. Materials & Methods: 24 adult mice (weighing 25-20 gr at 6 to 8 weeks of age) were divided equally into 4 groups in that group 1, control group, that received normal saline (0.1ml, IP).The group 2 was received phenylhydrazine (PHZ) with dose of 8mg/100 gr b. w, IP, IP at first time and followed by 6mg/100 gr b. w/48h, IP. The group 3 received vitamin E (100 IU/kg, IP) along with PHZ and the group 4 received only vitamin E(100 IU/kg, IP). After scarifying the animals by vertebral dislocation, testicular samples were processed and paraffin sections stained with H & E staining method and they were evaluated respecting factors such as morphometric parameters such as the thickness of the tunica albuginea, leydig and Sertoli cells, germinal epithelium height, diameter of seminiferous tubules, TDI, SPI, RI, as well as sperm parameters such as motility, viability, chromatin condensation and DNA damage in sperm. Results: It was revealed that Tubular Differentiation Index (TDI), Republication Index (RI), Spermiogenesis Index (SI), leydig and Sertoli cells, germinal epithelium height and diameter of seminiferous tubules were increased significantly in control and experimental groups compared to PHZ group (p<0.05). Finally, administrating vitamin E resulted in remarkable (P<0.05) enhancement in sperm parameters. Conclusion: These results indicate that vitamin E protective role on oxidative stress originated from Anemia with Phenylhydrazine.

testis tissue, sperm parameters, phenylhydrazine, vitamin E, mice