Identify Species of Dermatophytes Using Random Primers OPAA17 by AP-PCR Method

Objectives: Dermatophytes are closely related to keratinophilic fungi that invade the keratinzed tissues in humans causing dermatophytosis. Identification of dermatophytes with conventional methods is time- consuming and sometimes problematic because of similarities and variabilities of species. Genetic amplification has made rapid and perfect identification of dermatophytes possible. AP-PCR method is used for typing, but the aim of this research was evaluation of AP-PCR method for identification of dermatophytes and to find a suitable approach for a rapid distinguishing of dermatophytes. Method: Fifty-two isolates from 10 species of dermatophytes including: T. mentagrophytes (10), T. verrucosum (9), T. rubrum (5), T. tonsurans (3), T, violaceum (2), T. schoenleinii (1), M. gypseum (8), M. canis (4), M. ferrugineum (2), E.floccosum (8) were collected and confirmed with microscopic, macroscopic and biochemical tests. After DNA extraction, Molecular identification was done using AP-PCR (arbitrarily primed PCR) with random primer OPAA17. These primers amplified bands of different sizes in species of dermatophytes DNA. Results: All species of dermatophytes were recognized with a distinct DNA band patterns on gel agarose. The range of obtained bands was between 200 to 1400 bp for all dermatophyte except

Dermatophyte