Effect of Phenylhydrazine-induced Hemolytic Anemia on Morphological Changes of Renal Cortex in Adult Mice: Protective Effect of Vitamin E and Ethyl Pyruvate

Background and Aim : Phenylhydrazine (PHZ) as a well-known compound causes toxicity in different levels in tissues. The present study aimed to evaluate the probable protective effects of vitamin E and ethyl pyruvate against the tissue damages caused by PHZ in mice kidney. Methods : The adult male mice have been divided in eight groups with 8 mice in each group. Control group received normal saline (0.1ml/day, IP), sham group received PHZ (6 mg/100gr/48h, IP) and first experimental group received PHZ along with vitamin E (100 IU/kg/day, IP). Second experimental group received ethyl pyruvate (40 mg/kg/day, IP) along with PHZ, and third experimental group received vitamin E and ethyl pyruvate with dose same like previous groups along with PHZ. First negative control group received vitamin E, second negative control group received ethyl pyruvate and third negative control group received vitamin E and ethyl pyruvate with doses same like previous groups without the PHZ. After 35 days of treatment period serum and kidney tissue sampled for serological, histological and histomorphometrical study. Results : This study showed histological changes on proximal tubules of kidney in PHZ (sham) group, but ethyl pyruvate and vitamin E partially ameliorated those effects. Serological study also showed decreasing in total antioxidant capacity (TAC) and increasing of malondialdehyde (MDA) in sham group. Hemolytic anemia induced by PHZ showed significant effects on proximal tubules (P<0.05), but not on the other structures of the kidney. Conclusion : It seems that ethyl pyruvate and vitamin E as free radical inhibitors decreased the kidney toxicity induced by PHZ in mice.

Phenylhydrazine, Hemolytic Anemia, Ethyl pyruvate, Vitamin E, Renal, Mouse