Detection of protein A effects on immune system.

Background: Highly-purified form of the Staphylococcal protein A (SpA) has the capability, at very low concentrations, to down regulate activation of human B-lymphocytes and macrophages which are the key cells interfering tumefaction in determined autoimmune diseases. Materials and Methods: In a PBMC in vitro model, we investigated the effect of different doses of three highly-purified forms of protein A, in comparison of commercial drug, on the synthesis of selected cytokines after stimulation with lipopolysaccharide (LPS) in adult patients with active rheumatoid arthritis (RA) and healthy donors. Results: Protein A in therapeutic serum condensations significantly prevented the expression of a majority of the cytokines examined. Conclusions: Immune- moderating effects of Protein A were shown in clinically related concentrations, in low doses reflecting serum concentrations found generally in patients. Protein A potentiates the suppressive influences of commercial drug on cytokine synthesis.

protein A, immune, therapeutic, cytokine