Heavy metals affect growth, morphology and metabolism of plants in several ways. To reveal relationship between metal toxicity, oxidative stress and detoxification responses, the effects of four different heavy metals (Cd, Co, Pb and Ag) on Aeluropus littoralis was determined. So, one-month-old plantlets were treated with different concentrations (50 μM and 100 μM) of heavy metals for one week and the contents of photosynthetic pigments, phenolic compounds, proline, soluble proteins and the activities of enzymatic antioxidants (superoxide dismutase, guiacol peroxidase and catalase) as marker for oxidative stress were investigated. Survey results indicated that the amount of non-enzymatic antioxidant compounds (phenol and proline) significantly were increased in all treatments but a significant decrease was observed in photosynthetic pigments in the most treatments. Also, the accumulation of soluble proteins and increase in superoxide dismutase activity of which are respectively recognized as primary and secondary response to metal toxicity that can be pointed to the great capability of A. littoralis for accumulation and tolerance to excess amount of heavy metals. These results collectively show that different response in control and treated plants is due to induction oxidative stress and heavy metal toxicity in plants because of the accumulation of reactive oxygen species in treated plants.
کلید واژگان :enzymatic antioxidants; phenoilc compounds; proline; protein; SDS PAGE
ارزش ریالی : 600000 ریال
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