Polyclonal antiserum from mice immunized with extracellular proteins from Rhizoctonia solani inhibited
pectinase and cellulase activities in cell free culture supernatants of Rhizoctonia solani. Spleen mRNA from
these mice was used to construct a cDNA library from which antipectinase ScFv antibodies were isolated
using phage display techniques. Soluble ScFv antibodies produced by individual clones in Escherichia coli
inhibited polygalacturonase in the culture supernatants of a range of fungal pathogens, including ascomycetes,
basidiomycetes, and oomycetes. The soluble antibodies also inhibited maceration of potato tissue
by these pathogens
