Evaluating of in vitro fertilization (IVF) of mice with eperimental hemolytic anemia; protective effects of royal jelly

Corresponding Author: Rasoul Shahrooz, Department of Basic Sciences, School of Veterinary Medicine, Urmia University, Urmia, Iran, email: hojatanbara@gmail.com Background: Hemolytic anemia induced by phenylhydrazine (PHZ) as a hemolytic compound can cause reproductive disorders. The present study aimed to evaluate the royal jelly (RJ) as effective antioxidant compounds against PHZ-induced hemolytic anemia. Methods: This study was performed by 32 adult male mice in four groups. Group of control received 0.1ml normal saline intraperitoneally (IP). The PHZ group received 60 mg/kg, IP, PHZ in 48 hour intervals. The PHZ+RJ group received 100 mg/kg of royal jelly orally along with PHZ. The RJ group received only royal jelly similar to previous group. 35 days after treatment, the sperms were collected from epididymis and their in vitro fertilization (IVF) was evaluated. Result: Experimental hemolytic anemia significantly reduced the number of fertilized oocytes, two and fourcell embryos, morula, blastocyst and arrested embryos. However, in group that received PHZ along with RJ all of these parameters were ameliorated significantly (P Conclusion: It can be conclude that royal jelly as a free radical scavenger has a potential capacity to decrease oxidative damages on reproductive organ in experimental hemolytic anemia.

In vitro fertilization, Phenylhydrazine, Royal jelly, Hemolytic anemia