Development and application of new carbon paste electrode modified with copper aceylacetonate for amitriptyline determination

This work presents a voltammetric study using a modified carbon paste electrode for the evaluation of amitriptyline concentration in pharmaceutical preparations and attempts to optimize the analytical conditions. Amitriptyline (AMT) is a pharmaceutical belonging to the azepine group of compounds, and it is widely used due to its antipsychotic and sedative activity as well as analgesic properties. It controls psychomotor disturbances such as agitation, excitement, hyperkinetic states, and aggression. However, AMT may cause side effects including heart problems. Therefore, it is extremely important to analyze concentration levels of this chemical substance in commercial formulations and biological samples. Some electrochemical methods for determining AMT and other same-class psychotropic drugs have been reported in the literature.[1] Electrochemical methods may be used for this purpose due to their excellent sensitivity, short analysis time, simplicity, and the low costs involved in the implementation of these powerful tools for measuring different analysts. Herein, adsorptive stripping differential pulse voltammetry as a very sensitive analytical method was used. Effect of various parameters such as pH, amount of Cu(acac)2, accumulation potential and accumulation time was investigated. Amitriptyline, which was electroinactive with the normal carbon paste electrode, became electroactive on the modified electrode. Under optimum conditions, the calibration curve of anodic peak current versus amitriptyline concentration was linear in the range of 0.1-80 µM with a detection limit of 20 nM. Therefore, the developed method also was successfully applied for the AMT determination in real samples of pharmaceutical formulations and can be used for routine quality control analysis of bulk pharmaceutical and pharmaceutical formulations containing AMT

amitriptyline, Cu(acac)2, electrochemistry