Background: Radio therapy plays an important role in controlling tumor growth in esophageal cancer patients. Objectives: Our study provided comprehensive information about radio-sensitivity of Hdm2 gene in esophageal cancer cells in cancer cells. Methods: The expression of Hdm2 gene in radiosensitive and radio-resistant esophageal squamous cell carcinoma TE1, TE8 and TE11 cell lines is studied. RNA extracted from each esophageal cancer cell, then Quantitative reverse transcription PCR (RT-qPCR) assay were used for measuring HDM2 gene expression. Finally, radiosensitivity was determined for every three esophageal cancer cell lines. Results: It was found that Hdm2 gene significantly expressed in TE1, TE8 and TE11 cell lines by qRT-PCR analysis. Clonogenic assay showed that TE-11 was very sensitive to radiation relative to other cell lines. Conclusions: Hdm2 protein binding to the P53 protein is a key regulator of cell cycle control and genome protection Cell cycle control, programmed cell death and response cells to radition play important roles.
کلید واژگان :Esophagus Cancer, Hdm2, P53, Radiosensitivity, Squamous Cell Carcinoma
ارزش ریالی : 600000 ریال
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جزئیات مقاله
- کد شناسه : 2157763129524701
- سال انتشار : 2017
- نوع مقاله : پذیرفته شده در مجلات Scopus ,ISI, با IF=0
- زبان : انگلیسی
- محل پذیرش : International Journal of Cancer Management
- برگزار کنندگان :
- ISSN : 2538-4422, 2538-497X
- تاریخ ثبت : 1398/10/08 18:24:55
- ثبت کننده : جلیل پیرایش اسلامیان
- تعداد بازدید : 224
- تعداد فروش : 0