Ticks are the main cause of damage to livestock industries annually. Recently, anti tick recombinant vaccines have been developed by using a concealed antigen Bm86 against Boophilus microplus which is named Tick GARD and GAVAC in Australia and Cuba respectively. Using Single-step method, total RNA was extracted. RNA concentration was measured by spectrophotometry.Using oligo(dT) method RT-PCR was done and finally, after cDNA synthesis, using one pair of primer (155U19/ 515L19) PCR was done. A 266bp fragment at the N-terminal of cDNA was sequenced. This fragment was compared by Bm86 gene homologue in Iranian Hyalomma anatolicum anatolicum, and Bm86 gene in Boophilus microplus using BLAST and DNAMAN programs. A high homology for Iranian Hyalomma anatolicum. anatolicum (98%) and (80-82%) for
Boophilus microplus was recorded.
