To date, a fewnumbers of bacteriophages that infect Lactococcus garvieae have been identified, but their complete genome sequences have not yet been investigated. For the first time, herein, the complete DNA sequence of a new phage of L. garvieae (phageWP-2) is reported and analyzed. Themorphological characteristics indicated that the phage had a small isometric head alongwith a short and non-contractile tail, suggesting thatWP-2 belongs to the family Podoviridae. Bioinformatic analysis revealed that phage WP-2 can be classified as a new member of Ahjdlikevirus in the Picovirinae subfamily because it had a small dsDNA of 18,899 bpwith 24 open reading frames and a protein-primed DNA polymerase. The phage nucleotide sequence and predicted protein products have been identified to share very limited evidence of homology with complete genome and proteome of other phages. To our knowledge, this is the first Ahjdlikevirus bacteriophage which can infect a member of the Lactococcus genus.
کلید واژگان :Bacteriophage, Podoviridae, Picovirinae, Ahjdlikevirus, Phi29likevirus, Lactococcus garvieae
ارزش ریالی : 600000 ریال
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