Lactococcosis, a significant emerging disease of fish caused by Lactococcus garvieae, has become one of the devastating problems due to its serious economic damage in aquaculture. The aim of this study was to isolate and characterize a lytic phage infecting L. garvieae as a potential bioagent for the treatment of lactococcosis. In this regard, one strain of L. garvieae was isolated from diseased rainbow trout, and then, following biochemical and molecular identifications, its specific phage,WWP-1, which was able to destroy L. garvieae cells through the lytic cycle,was isolated fromamunicipalwastewater sample. Transmission electronmicroscope revealed that the isolated phage possesses an icosahedral head and a non- contractile short tail, resembled to members of the family Podoviridae. Moreover, phage WWP-1 represented optimal antibacterial activity at temperatures ranging from 15 to 30 C, suggesting that it could be very effective at rainbow trout rearing temperature. Restriction profile analysis revealed that NdeI can digest WWP-1 genome while EcoRI, EcoRV, and BamHIwere incapable of cutting itsDNA.According to the in vivo experiment result,WWP- 1 could decrease mortality rate of infected rainbow trout in aquaculture. The results suggest that this naturally occurring bacteriophage could be considered as a promising agent to control the disease caused by L. garvieae strains in rainbow trout rearing.
کلید واژگان :Aquaculture, Bacteriophage, Lactococcus garvieae, Lactococcosis, Phage therapy
ارزش ریالی : 600000 ریال
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