Calendula sp. is an important medicinal and industrial plant with various bioactivities. The field study was conducted in Jiroft region of Kerman, Iran, to observe the effects of gibberellic acid (GA3) on phenolic compounds and antiradical activity of Marigold (Calendula officinalis) extract. The levels of GA3 were 0, 50, 150 and 250 mg L-1 in three spraying stages (first, second and third). The phenolic content was measured by the Folin–Ciocalteau method and the antioxidant activity by DPPH (2, 2-diphenyl-1-picrylhydrazyl) method. Results of this study showed that application of GA3 at first stage and second stage (first and second) significantly increased the content of phenolic compounds in C. officinalis (61.96 and 62,74 mg gallic acid/100 g dry weight respectively) while at third stage decreased the phenolic compounds. In all spraying stages, by increasing of GA3 concentration, phenolic compounds of C. officinalis extracts were increased. Antiradical activity of C. officinalis extracts showed effects similar to the phenolic compounds. C. officinalis had phenolic compounds and scavenged free radicals in-vitro and using of different GA3 levels could increase its phenolic compounds and antiradical activity.
کلید واژگان :Calendula officinalis, gibberellic acid, phenolic compounds, antiradical activity.
ارزش ریالی : 600000 ریال
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