Hepatitis C virus (HCV) is the etiologic agent of most parentally transmitted hepatitis viruses and is responsible for more than 60% of chronic hepatitis cases that lead to liver transplantation. Based on its genetic variability, HCV is classified into at least six genotypes and a series of subtypes. HCV genotyping is important in order to select appropriate therapy. The aim of this study was to determine which HCV virus genotypes are most prevalent in Tehran. Serum HCV RNA was extracted by RNXplus buffer. RT-PCR and nested PCR were used on the 5’ untranslated region and a 250 bp fragment was amplified. 80 HCV positive sera samples were selectively chosen from 250 patients who were referred to the laboratory by their physicians. HCV genotypes were determined using restriction fragment length polymorphism (RFLP). The patients were grouped as follows: 32 cases (40%) had type 1a, 15 cases (18.75%) had type 3a, 7 cases (8.75%) had type 1b, 5 cases (6.25%) had type 3b, 3 cases (3.75%) had type 4, and 18 cases (22.5%) could not be typed because their sequences differed from any that were previously reported. Our results showed that types 1a and 3a were the most prevalent HCV genotypes in our Tehran samples, while types 2a, 2b, 5, and 6 were not observed.
کلید واژگان :Hepatitis C virus, genotyping
ارزش ریالی : 600000 ریال
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