Toxoplasma gondii is an obligate intracellular parasite which its sexual and asexual cycle respectively takes place in
the intestinal epithelial of definitive host and tissue of intermediate hosts. Congenital toxoplasmosis is more
important when the mother acquired the infection during pregnancy period for the first time. Having a specific
antigen is an important element in prevention and detection of parasite. This study has designed and performed in
the aim of cloning a specific toxoplasma antigen for further studies. We have amplified gene of P43 of toxoplasma
tachyzoite and bradyzoite surface antigen. PCR product was cloned in pGEMEX1 expression vector (named
pGEM43) and is ready to make the recombinant protein for using as antigen.
