Novel ESAT -1081 multiplex PCR for direct detection and identification of BCG strains from other members of mycobacterium tuberculosis complex

Bacille Calmette Guerin (BCG) was initially used as vaccine against tuberculosis, however, it uses for verity of clinical application. Detection of adverse effects and complications of BCG is essential for necessary timely quality control of the vaccine, administration techniques and its application Direct microscopy and culture are still the methods of choice for detection of BCG in most laboratories, but they are very difficult processes and time-consuming. An ESAT-IS1081 multiplex PCR amplification of targeted gene segments was optimized to detect and differentiate the BCG strains from other members of M. tuberculosis complex. The sensitivity of detection for H37Rv, M. bovis and BCG were dilution of 104,105 and 105 cells mL-1 of cell stock suspension, respectively. The results from this study showed that the ESAT-IS1081 multiplex PCR assay permits a specific, sensitive and reproducible system for the detection and differentiation of the BCG, thereby improving the clinical management of BCG complication to clinician.

BCG, ESAT-IS1081, multiplex PCR