Sheep are the most abundant small ruminants in Iranian rural communities. Zoonotic infections and diseases are limiting factors for the fast growth of the sheep industry in Iran, yet surveillance during meat inspection at the slaughter houses is not routinely done. The aim of the study was two folds; 1) to determine the prevalence of Mycobacterium tuberculosis (M. tuberculosis), Brucella abortus (B. abortus) and Actinomyces pyogenes (A. pyogenes) in the testicles of rams from the Isfahan province in central Iran, 2) to determine the predilection sites of these bacteria in the infected testicles. A total of 100 pairs of testicles were randomly collected from different slaughter houses within the Isfahan province, transported to Azad University at +4°C until further analyses. IS6110, bcsp31K gene and 16S rDNA of M. tuberculosis, B. abortus and A. pyogenes were detected in PCR reactions respectively. The prevalence of the M. tuberculosis was higher (13%) than those of B. abortus (8%) and A. pyogenes (5%). The present study revealed a higher prevalence of the M. tuberculosis than once reported elsewhere in sheep. The findings add to the existing knowledge that sheep may be susceptible to B. abortus in addition to Brucella ovis and Brucella melitensis. The need for proper screening before and after slaughtering is very important for control of these zoonoses.
کلید واژگان :Prevalence, Rams, Mycobacterium, Brucella, Actinomyces, Iran
ارزش ریالی : 600000 ریال
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