Mutations in bone morphogenetic protein-15 (BMP-15) gene can increase ovulation rate and infertility in sheep. Polymorphisms in BMP15 gene of Najdi and Lori-Bakhtiari goats using PCR-SSCP is described. 117. From genomic DNA, 235 bp fragments of exon 2 of BMP15 gene were amplified by PCR with specific primers and assayed by single stranded conformation polymorphism. AA, BB, FF, DD, EE, AD and AB genotypes with a frequency of 0.155, 0.167, 0.011, 0.011, 0.123, 0.133 and 0.4 respectively, were detected in Najdi goat and AA, BB, CC, DD, AD and AB with a frequency of 0.259, 0.075, 0.037, 0.037, 0.185 and 0.407 respectively in Lori baktiari goat. Heterozygosity was 0.533 and 0.593 respectively in Najdi and Lori-Bakhtiari goats. BMP15 gene is associated with folliculo genesis in sheep and thus is a candidate gene for litter size in goats.
کلید واژگان :Morphogenetic Protein 15, Goat, Prolificacy, PCR-SSCP
ارزش ریالی : 600000 ریال
با پرداخت الکترونیک