بررسی اثر دوزهاي مختلف كافيين و يك جلسه فعاليت ورزشي فزاينده بر استرس اكسايشي و آنتي اكسيدان هاي غير آنزيمي مردان فعال

Assessment of different Caffeine doses and one Session incremental exercise Effect on oxidative stress and nonenzymatic antioxidant status in active men the aim of this study was to assess of different caffeine doses and one session incremental exercise effect on oxidative stress measuring serum GSH, vitamin E, Uric acid, Lactate and malonaldehyde (MDA) as markers of nonenzymatic antioxidant status and lipid peroxidation respectively. 10 men students of physical education were selected and participated in a double blind (caffeine doses 5 or 9 mg/kg body weight or placebo) treadmill test until exhaustion. Test was performed in separate 3 days and each subject was underwent one of caffeine doses or placebo conditions in every session. Venous blood samples were obtained for analysis before of exercise and caffeine doses / placebo ingestion(Pre) and immediately after the test(Post). Paired T test and one-way ANOVA test and LSD-post Hoc test were used to statistics analysis for data in signification level loss (P< 0/05). Statistical analyses were done using SPSS/13.5. results showed significant decreas in Experimental groups for MDA (P<0/05) whereas these modifications in joint to significant increases for GSH and Lactate were determined (P<0/05). Although 5 mg/kg caffeine had no significant effect on serum vitamin E and uric acid amounts (P > 0/05). 9 mg/kg caffeine dose significantly increased vitamin E and uric (P <0/05). There were no significant difference between experimental groups for MDA and uric acid (P > 0/05), but these differences for GSH, vitamin E and lactate were significant (P <0/05). There were significant differences for MDA, GSH and uric acid between experimental groups (P <0/05). Although these differences for vitamin E and lactate were not significant between 5 mg/kg caffeine and placebo groups (P > 0/05). In conclusion different caffeine doses decreased sport oxidative stress and effectively augmented nonenzymatic antioxidant status. it seems 9 mg/kg caffeine dose in comparison of 5 mg/kg more effectively increases nonenzymatic antioxidant status after incremental exercise. Keywords: Incremental exercise, Lipid Peroxidation, malonaldehyde, GSH.