چکیده :

NeisseriameningitidesisaGramnegativebacteriumwhichisanimportantcausativeagentofsepticemiaand meningitis.NumerouspathogenicbacteriacontainluxS,whichisrequiredforautoinducer2production.Neis seriameningitidescontainsafunctionalcopyofluxSthatisnecessaryforfullmeningococcalvirulence.Neisse riameningitidesDNAwasextractedanditsluxSgenewasamplifiedbynestedPCR.PCRproductwaspurified andclonedintopQE30expressionvector.Recombinantplasmidwastransformedandmasscultured.luxSwas expressedinE.coliandconfirmedbywesternblotanalysis.Inthisstudy,N.meningitidesluxSwasamplified, clonedandexpressedsuccessfully.ThesequencingofPCRproductconfirmedthatamplifiedgenewasluxS. GenewasexpressedandobservedinSDSPAGE.Proteinwasreactedbyhistagmonoclonalantibodythrough westernblotanalysis

کلید واژگان :

Neisseriameningitides;luxS;autoinducer2;clonin



ارزش ریالی : 600000 ریال
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