The purpose of this study was to establish best condition for in vitro propagation of thornless trailing blackberry (Rubus sp.). Axillary buds were used as explants. After sterilization, explants were placed into MS medium supplemented with 2 mg.l-1 BA. Two weeks after that, in shoot proliferation stage, 12 culture media containing MS supplemented with 3 concentrations of BA (0, 2 and 3 mg.l-1), alone or in combination with GA3 (0, 0.2, 0.5 and 1 mg.l-1) were compared. The greatest number of shoots with average of 3.33 and the maximum shoot length with average of 5.87 cm were produced in medium containing 2 mg.l-1 BA and 0.5 mg.l-1 GA3 . In the rooting experiment, rooting medium comprised MS medium in combination with different concentrations of IBA (0, 0.5, 1 and 2 mg.l-1). A concentration of 2 mg.l-1 IBA gave a greater number of roots and maximum root length. In this medium four roots with root length average of 7.83 cm were produced
کلید واژگان :axillary bud; blackberry; in vitro; micropropagation
ارزش ریالی : 600000 ریال
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