In the present study, conventional and real-time PCR assays were developed in order to detect bovine herpes virus type 1 in clinical samples. A total of 16 (11.18%), 10 (11.11%) and 8 (10.12%) aborted bovine, buffalo and camel foetuses were respectively positive for the specific amplicon of 161 bp fragment for BHV-1 DNA in conventional PCR. After using real-time PCR, the glycoprotein B gene of BHV-1 was seen in 21 (14.68%), 14 (15.55%) and 10 (12.65%) of aborted bovine, buffalo and camel foetuses, respectively. There was no significant difference between the abilities of conventional and real-time PCR assays for detection of BHV-1 in abomasal contents of aborted foetuses. Statistical analysis showed significant differences (P<0.05) between buffalo and camel aborted foetuses with regard to the presence of the virus. The Ct values obtained from real-time PCR for presence of BHV-1 showed significant differences (P<0.05) between buffalo and camel aborted foetuses. Our results showed that the real-time PCR assay was 2 times more sensitive than the conventional PCR. The Gilan province had the highest BHV-1 prevalence and Kordestan: the lowest. Based on the results of this study, camels were the most resistant and buffaloes – the most sensitive to abortions caused by BHV-1. This is the first prevalence report of direct detection of BHV-1 in aborted bovine, buffalo and camel foetuses by evaluation of conventional and real-time PCR in Iran.
کلید واژگان :aborted foetuses, BHV-1, conventional PCR, real-time PCR
ارزش ریالی : 600000 ریال
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