A simple graphical linear method was introduced for isothermal titration calorimetric data analysis in the protein-ligand interaction.
The number of binding sites, the dissociation binding constant and the molar enthalpy of binding site can be obtained by using this
new isothermal titration calorimetric data analysis method. The method was applied to the study of the interaction of human growth
hormone (hGH) with divalent calcium ion at 27°C in NaCl solution, 50 mM. hGH has a set of three identical and independent binding
sites for Ca
2+
. The intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 52 μMand –17.4, respectively.
Results obtained by this new calorimetric data analysis are in good agreement with results obtained using our previous method
کلید واژگان :
calcium, calorimetric method, human growth hormone, isothermal titration calorimetry
