NAD(P)H: quinone oxidoreductase (NQO1) plays an
important role in detoxification of numerous endogenous
and foreign compounds. This gene has a single
nucleotide polymorphism at site of codon 187
(CCT TCT). Recently, it has been demonstrated that
individuals with T allele may exhibit resistance to
quinone based anticancer drugs such as mitomycin C.
In the present study, a simple and feasible method was
developed for detection of NQO1 genotype. In this
modified procedure, dimethylsulfoxide (DMSO) and
Triton X-100 were eliminated, also, PCR cycling conditions
were modified to improve the PCR products from
blood and formalin-fixed, paraffin-embedded tissues.
PCR-RFLP and DNA sequencing analysis carried out
on a limited number of blood and archival samples. It
is suggested that this procedure convenient for NQO1
genotyping.
