To determine the disease status of Iranian rainbow trout aquaculture to viral haemorrhagic septicemia (VHS), research samples were collected from farmed rainbow trout populations that had symptoms of VHS disease. Fish were sampled between October 2004 and October 2006, from 100 rainbow trout sites in 10 provinces in Iran. Samples for diagnostic screening were aseptically collected from liver, kidney, spleen, bronchia, heart, intestine, and pancreas of rainbow trout with symptoms of VHS disease and all were screened by both molecular and pathological techniques. The first confirmed VHS disease outbreaks occurred in Iranian aquaculture in November 2005 at Rodsar of Gilan province in the north of the country. In this study of 100 rainbow trout fish farms, 15 positive sites were identified by pathology and 10 farms screened VHSV positive by nested-PCR. The results of this study reveal a potential high frequency of VHS virus in some centers of rainbow trout aquaculture in Iran and therefore the control and diagnosis of VHS disease is vitally important to the development of the Iranian rainbow trout aquaculture industry. This is the first study of VHS infection in rainbow trout aquaculture sites in Iran and highlights the need for routine diagnostic screening of aquaculture facilities to control the spread of VHS disease.
کلید واژگان :viral haemorrhagic septicaemia , nested-PCR,
ارزش ریالی : 600000 ریال
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