A partial DNA library of kinetoplast DNA (KDNA) from leishmania major (MRHO/IR/64/nadim-1) was prepared in Bluscript plasmid. Using Dot blot and Southern blot analysis against three different leishmania strains, L. infantum, L. major and L.tropica , one of them (IRI-m1), showed to be specific for L.major (MRHO/IR/64/nadim-1) and the other , IRI-m2 hybridized with L.infantum as well as L.major. Both of these tow fragments were sequenced. It was shown that these tow fragments possess only 56% sequence homology. These finding denote that heterogeneity of minicircle DNA exist in a sigle species.
کلید واژگان :
Leishmania, kinetoplast DNA, minicircle, DNA probe, Riboprobe
