Susceptibility to fluconazole in Candida species isolated from vulvovaginal candidiasis in Ahvaz

Introduction and Objectives: Vulvovaginal candidiasis is considered as a common opportunistic mycosis in women genital system in the world. Disease is usually associated with considerable morbidity, healthcare cost, distress, pain and sexual functioning. Candida albicans is the most agent of Candida vaginitis followed by C. glabrata and C. tropicalis. Fluconazole is a triazole antifungal that are used for the treatment of Candida vaginitis as a single dose. Several reports shows that the incidence resistant to fluconazole rising during two last decade. In the present study, susceptibility Candida vaginitis isolates evaluated against fluconazole. Materials and Methods: Vaginal samples were collected using sterile cotton swabs and inoculated on CHROMagar Candida. Cultured media were incubated at 30-35°C for one week, aerobically. Colonies producing a green coloration were presumptively identified as Candida albicans. In addition, this species was confirmed by germ tube and chlamydoconidia formation. Other species were detected using CHROMagar Candida, ID 32C and API 20C AUX kits. Susceptibility to fluconazole was determined using ATB Fungus 3 kit. Results: In the present study C. albicans (47.2%) was the most common species among the isolates followed by C. glabrata (24.1%), C. dubliniensis (12%) and Candida species (5.6%). In the present study resistant to fluconazole was found in 34.8% of isolates. C. dubliniensis was the most common isolate (6 of 11 isolates) that show resistant to fluconazole. Conclusion: Based on the present study, resistant to fluconazole among vulvovaginal candidiasis agents is considerable. In addition, this resistant was more common against C. dubliniensis than other species of Candida.

Vulvovaginal Candidiasis, Candida albicans, Fluconazole