Background: Toluene is a cyclic aromatic hydrocarbon which is widely used as an industrial feedstock and as a solvent. It is one of th major parts of pollution in oil-contaminated environments. Objectives: The main aim of this study was to isolate and characterize a Bacterium with high potential application in toluen bioremediation. Materials and Methods: To isolate a toluene-degrading Bacterium, several seawater and wastewater samples were added toluene-containing basal salt media (BSM). The isolate was identified by morphological features, biochemical tests, and molecul characterization. Also, physiological characteristics of the isolated strain were determined. Results: The isolate represented the capability of growing on toluene under both aerobic and anaerobic conditions. Moreover, th Bacterium could also use different toxic compounds as the sole sources of carbon and energy. Sequence analysis of 16S rDNA showe that the isolated strain was closely related to Uncultured Bacterium clone A1-E3_M13R (98%) and was submitted as Bacterium Ex-DG7 in NCBI. Bacterium Ex-DG74 showed a tolerance to organic solvent and saline conditions as it could grow in the medium containin over 15% toluene (v/v) and NaCl (w/v), and degraded 79% and 45% of toluene (1% (v/v)) in aerobic and anaerobic conditions, respective Conclusions: In this investigation we succeeded to isolate a novel toluene-degrading Bacterium from wastewater. This isolated stra could be considered as a biological material for the toluene bioremediation.
کلید واژگان :Bacterium Ex-DG74; Bioremediation; Gas chromatography; Toluene
ارزش ریالی : 600000 ریال
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