The fg (Ficus carica L.), is known as a precious fruit tree for its nutrition and medicinal values, economic importance and for sustainable production in the semi-arid and arid areas. Expanding the cultivation of fg in new vulnerable areas and the breeding programs in fg need a reliable high-efcient system for in vitro morphogenesis to meet future demands. This study was carried to develop an efcient protocol for indirect regeneration of F. carica L. cultivars ‘Sabz’ and ‘Torsh’ using thin cell layer (TCL) technique. The genetic fdelity of the regenerated plants was also evaluated using fow cytometry technique and ISSR markers. Stem segments of 10 mm in diameter were taken from mature plants, then explants were transversally cut into layers of 0.5–0.8 mm thickness. Callus induction was successful using Murashige and Tucker (MT) medium supplemented with 9.08 μM TDZ plus 9.8 μM IBA (IM3 medium) which resulted in 50±6.11% calli in ‘Sabz’ cultivar. Morphogenic calli were cut into small pieces and cultured on Murashige and Skoog (MS) medium for shoot development. Maximum shoot regeneration (45%) was observed in 17.68 μM BAP in combination with 4.54 μM TDZ and 1.07 μM NAA (RM2 medium), with an average of 6.9 shoots per explant. Flow cytometry and ISSR molecular marker analyses confrmed the stability of ploidy level and genetic identity of indirectly regenerated plants in both cultivars. The results of this study demonstrate that indirect regeneration of F. carica L. by the use of TCL system is a reliable and promising approach for future mass propagation programs as well as possible in vitro breeding objectives.
کلید واژگان :Fig (Ficus carica L.) · Flow cytometry · Molecular markers · Morphogenic callus · Plant growth regulators
ارزش ریالی : 600000 ریال
با پرداخت الکترونیک