Background: Toxoplasmosis is a serious disease in immunocompromised patients and pregnant
women. Differentiation of acute and chronic infection is a major challenge in serodiagnosis of the
disease. Since the aim of this study was to assess the diagnostic utility of recombinant SAG1 (rec- SAG1) for the detection of Toxoplasma-specific IgM antibodies in human sera, by an enzyme-linked
immunosorbent assay (ELISA).
Methods: The purified recombinant protein SAG1 was applied in house ELISA test and the ability
of it in binding to specific immunoglobulin M in 30 serum samples of acute infected patients was
evaluated. The results obtained by assays with the recombinant SAG1 and standard commercial assays
were compared.
Result: The sensitivity and specificity of in house ELISA compared to a standard commercial
ELISA (com-ELISA) were 80% and 90%, respectively.
Conclusion: It was concluded that the rec-SAG1 could be an alternative marker for detection of
anti Toxoplasma-specific IgM and diagnosis of acute infection.
