This study was to evaluate the effect of a preparation of Bacillus probiotic (Bacillus licheniformis and B. subtilis, 1:1) on growth and survival rate of Pacific white shrimp, Litopenaeus vannamei larvae. The larvae were fed on Artemia urmiana nauplii and Brachionus plicatilis enriched with the probiotic preparation at 1 9 106 CFU mL-1 rate. The experimental setup was completely randomized design comprised of six treatments, namely solo Artemia nauplii (A) or rotifer (R), Artemia nauplii and rotifer without any enrichment (A ? R), Artemia nauplii enrichment with probiotic bacilli (Bacillus licheniformis and B. subtilis) (A ? B), rotifer enrichment with probiotic bacilli (R ? B) and enriched Artemia nauplii and rotifer (A ? R ? B). All treatments were performed in triplicate. Chemical parameters of rearing water viz. pH, salinity and temperature were 7.5–8, 30–31 ppt and 31–32 C, respectively. Photoperiod was 16L:8D. Shrimp larvae were fed Artemia nauplii and rotifers at 5–20 and 10–40 individuals per shrimp larvae four times a day, respectively. Growth and survival rate of larvae were determined at MII, MIII, PL1, PL4, PL7 and PL10 stages. Larvae in A ? R ? B treatment showed the highest total length (10.89 ± 0.51 mm), weight (674 ± 73 lg) and survival rate (65 % ± 3.5). Lowest total length, weight and survival rate (7.96 ± 0.63 mm, 493 ± 52 lg and 24.5 ± 2.4 %, respectively) were recorded in treatment B larvae. We concluded that Bacillus probiotic can improve growth and survival rate of Pacific white shrimp larvae without conceivably undesirable effects.
کلید واژگان :Bacillus, Shrimp, Litopenaeus vannamei, Artemia urmiana, Brachionus plicatilis
ارزش ریالی : 600000 ریال
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