The population genetics of the Afghan Pika (Ochotona rufescens) was studied in Northern Khorasan Province, Iran. For prediction of the genetic differentiation of four populations, the DNA of mitochondrial cytochrome b of 32 individuals from four areas was sequenced and a Bayesiananalysis based on the HKY model was constructed. In total, 15 polymorphic sites,1125 conserved sites (98.7%) and 14 different haplotypes were found. The phylogenetic tree resulting from the Bayesian analysis and network analysis showed that all samples were clustered in two major groups and the haplotypes of the four populations did not separate geographically. An analysis of molecular variance (AMOVA) indicatedthat a large majority of the genetic variance was due to the variance within populations. The results of fixation indices showed significant genetic structure among populations in both methods. The pairwise Fst revealed that two northern populations have a significant genetic differentiation from two southern populations, but no significance pairwise Fst value was demonstrated between the closed populations. Nei’s geneticdistances between closed populations were not significant, while significant values occurred between distant populations. It seems that there is not a major discontinuity between populations of Afghan Pika based on cyt-b mitochondrial gene. However, phylogenetic analysis did not separate populations and a large majority of the genetic variance was found within populations. However, AMOVA analysis showed a significant level of genetic structure among populations (p<0.001) and between groups (p<0.5). It seems that these results suggest shallow genetic differentiation between populations of different geographic groups.
کلید واژگان :Ochotona rufescens; cytochrome b; genetic differentiation; population.
ارزش ریالی : 600000 ریال
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