An extraction method based on aptamer sorbent followed by electrospray ionization-ion mobility spec-
trometry (ESI-IMS) has been developed for the analysis of tetracycline in human urine and plasma
samples. The effect of extraction parameters on the extraction efficiency including washing (solvent
type and volume) and elution (solvent type, volume and flow rate) were investigated. Under the opti-
mized conditions, the linear dynamic ranges for tetracycline in urine and plasma samples were found to
be 0.05–5.00 g/mL and 0.10–5.00 g/mL with detection limits of 0.019 and 0.037 g/mL, respectively.
The
extraction
efficiency
was
86.5%
for
urine
and
it
was
82.8%
for
plasma
samples.
The
relative
standard
deviation was 5.9% and 6.3% for six replicate measurements of tetracycline at 1 and 2 g/mL in urine and
plasma samples, respectively.
کلید واژگان :
Tetracycline, Aptamer, Ion mobility spectrometry, Solid-phase extraction
