Salmonella is one of the important bacteria in egg and as major source in bacterial infection transfer to human and animals. It has been proven that gyrA gene can mediate in resistance and reduced susceptibility to fluoroquinolones, therefore the aim of the present study was to determine gyrA gene polymorphism in S. enterica ser. Enteritidis isolated from egg shell using PCR-RFLP assay. A total of 300 samples of egg shells were collected from seven aviculture in Chaharmahal Va Bakhtiari province and genomic DNA was extracted using DNA extraction kit. PCR-RFLP assay was used to identification of gyrA gene polymorphism using HinfI and BanI restriction endonuclease enzymes. A total of 85 ser. Enteritidis was detected from 300 samples and gyrA gene was isolated from all 85 positive ser. Enteritidis that out of 85 gyrA gene, 23 samples (27%) and 25 samples (29.4%) were respectively positive for HinfI and BanI enzymes. The results of the present study showed a relatively low prevalence of ser. Enteritidis infection in egg shells, but the rate of gyrA gene mutation was high that is very disturbing.
کلید واژگان :SALMONELLA ENTERICA
ارزش ریالی : 600000 ریال
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